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Literature summary extracted from

  • Medley, Q.W.; Lee, S.F.; Cote, G.P.
    Purification and characterization of myosin II heavy chain kinase A from Dictyostelium (1991), Methods Enzymol., 196, 23-34.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.7 additional information enzyme is stimulated by its autophosphorylation Dictyostelium discoideum

General Stability

EC Number General Stability Organism
2.7.11.7 loses about 25% of activity for every freeze-thaw cycle Dictyostelium discoideum
2.7.11.7 phosphorylated enzyme is somewhat less stable than unphosphorylated enzyme Dictyostelium discoideum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.7 additional information ionic strength has no effect on autophosphorylation activity; strong inhibition of phosphorylation activity by increasing ionic strength Dictyostelium discoideum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.7 0.1
-
 RKKFGESEKTKTKEFL-amide pH 7.5, 25°C Dictyostelium discoideum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.7 Mg2+ requirement Dictyostelium discoideum
2.7.11.7 Mg2+ absolutely requires 1-2 mM Mg2+ Dictyostelium discoideum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.7 130000
-
 x * 130000, SDS-PAGE Dictyostelium discoideum
2.7.11.7 700000
-
 above, gel filtration Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.7 ATP + myosin I heavy chain Dictyostelium discoideum
-
 ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain Dictyostelium discoideum Ax-3
-
 ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin II heavy chain Dictyostelium discoideum
-
 ADP + myosin II heavy chain phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.7 Dictyostelium discoideum
-
-
-
2.7.11.7 Dictyostelium discoideum
-
 isozyme myosin II heavy chain kinase A
-
2.7.11.7 Dictyostelium discoideum Ax-3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.7 isolation of highly phosphorylated and unphosphorylated myosin II heavy chain kinase A Dictyostelium discoideum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.7 cell culture
-
 Dictyostelium discoideum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.7 0.98
-
 purified enzyme Dictyostelium discoideum
2.7.11.7 0.98
-
 substrate myosin II heavy chain Dictyostelium discoideum

Storage Stability

EC Number Storage Stability Organism
2.7.11.7 0°C, highly purified preparation of unphosphorylated enzyme, 25% loss of activity per day Dictyostelium discoideum
2.7.11.7 0°C, partially purified preparation of unphosphorylated enzyme, several days Dictyostelium discoideum
2.7.11.7 indefinitely stable upon storage in liquid nitrogen, recovery of 60-70% activity after thawing Dictyostelium discoideum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.7 ATP + myosin heavy chain kinase intramolecular autophosphorylation Dictyostelium discoideum ADP + myosin heavy chain kinase phosphate
-
 ?
2.7.11.7 ATP + myosin heavy chain kinase enzyme incorporates 2-3 mol phosphate per mol of 130 kDa subunit Dictyostelium discoideum ADP + myosin heavy chain kinase phosphate
-
 ?
2.7.11.7 ATP + myosin heavy chain kinase leads to activation of the phosphorylation activity towards myosin I Dictyostelium discoideum ADP + myosin heavy chain kinase phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain
-
 Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain phosphorylation of residues Thr1833 and Thr2029 Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain phosphorylates threonine residues Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain 10 mol phosphate per mol of kinase subunit Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain 6-8 mol phosphate per mol of enzyme Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain
-
 Dictyostelium discoideum Ax-3 ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain phosphorylation of residues Thr1833 and Thr2029 Dictyostelium discoideum Ax-3 ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain phosphorylates threonine residues Dictyostelium discoideum Ax-3 ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain 10 mol phosphate per mol of kinase subunit Dictyostelium discoideum Ax-3 ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain 6-8 mol phosphate per mol of enzyme Dictyostelium discoideum Ax-3 ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin II heavy chain
-
 Dictyostelium discoideum ADP + myosin II heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin II heavy chain specific isozyme A for the heavy chain of myosin II Dictyostelium discoideum ADP + myosin II heavy chain phosphate
-
 ?
2.7.11.7 ATP + RKKFGESEKTKTKEFL-amide isozyme myosin II heavy chain kinase A Dictyostelium discoideum ?
-
 ?
2.7.11.7 ATP + RKKFGESEKTKTKEFL-amide synthetic peptide MH-3 Dictyostelium discoideum ?
-
 ?
2.7.11.7 ATP + synthetic peptides
-
 Dictyostelium discoideum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.7 ? x * 130000, SDS-PAGE Dictyostelium discoideum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.7 25
-
 assay at Dictyostelium discoideum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.11.7 25
-
 1-2 h, stable Dictyostelium discoideum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.7 7 7.5
-
 Dictyostelium discoideum

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.7 ATP
-
 Dictyostelium discoideum